Polymerase chain reaction, or PCR, is a laboratory technique ... the temperature of the sample in automatic, programmed steps. Initially, the mixture is heated to denature, or separate, the ...

Polymerase chain reaction ... and quantifies DNA as the reaction progresses. This quantifies the specific amount of DNA present in a sample. The procedure requires specialized thermal cyclers with ...

When the first thermal cyclers for the polymerase chain reaction came out in the ... PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands ...

2| Remove 48 μl to serve as the no-template control reaction and add 2 μl of primer pair 1. 3| Add 5.5 μl of genomic DNA at 10 ng/μl to the master mix (Step 1) and mix thoroughly. 4 ...