Download this application note from Thermo Fisher Scientific to discover an approach using the Neon NxT system that researchers can follow for optimal transfection results. Lab safety rules dictate ...

The work addresses the role of translation initiation in the early induction of specific genes during T cell activation. It primarily uses the Jurkat T cell line and demonstrates that the translation ...

To determine the role of Bid in apoptosis, EPC cells seeded overnight in six-well plates at 1 × 10 6 cells per well were transiently transfected using Lipofectamine 2000 (Invitrogen, USA) with 2 μg ...

In brief, HEK293T cells were plated on coverslips and were transiently transfected using lipofectamine 2000 transfection reagent (ThermoFisher Scientific) with plasmids expressing the following ...

At 48 h post-transfection, the cells were stimulated with ultrapure ... Macrophages were transfected with each siRNA by using Lipofectamine 2000 TM for up to 48 h, and then stimulated with LPS for 3 ...