CRISPR-Cas9 is a reprogrammable DNA cutting machine that is being used to edit genomes in many organisms for research purposes. Its primary component, the Cas9 enzyme (orange), cuts genomic DNA ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

This new tool, known as minimal, versatile genetic perturbation technology (mvGPT), combines the powers of gene editing, ...

Despite its successes, the CRISPR-Cas9 system has several limitations, including off-target cutting. Over the years, scientists have explored alternatives to Cas9, such as Cas12a for more precise ...

Gene editing involves precise changes in DNA sequences using enzymes, revolutionizing medical, agricultural, and research fields globally.

With vasa-Cas9, high efficiency and self-limiting suppression was achieved. Applying the CRISPR-Cas9 system to edit high GC content genomes has been challenging due to its high off-target activity.

Using CRISPR/Cas9 system to generate Osics mutants in rice and characterize the function of OsICS in the biosynthesis of phylloquinone and SA. Disclaimer: AAAS and EurekAlert! are not responsible ...

CRISPR-Cas9 editing leads to widespread loss of the targeted chromosome in human T cells, but scientists recently discovered a way to prevent such loss. To investigate how Cas9 gene editing affected T ...

Among his recent work at Drexel is the development of technologies based on the CRISPR-Cas9 system for genome mapping and sequencing, which have broad applications in molecular diagnostics, cell/gene ...

Intellia Therapeutics, Inc. is a clinical stage genome editing company, which engages in the development of curative therapeutics using the CRISPR/Cas9 system. Its CRISPR/Cas9 system transforms ...