Right: typical optical spectrum of HÜBNER Photonics’ VALO Femtosecond Series lasers. (Courtesy: HÜBNER Photonics) “If you decrease the pulse duration by a factor of five, this gives roughly a five ...

Two-photon calcium imaging was performed using custom-built microscopes (Leinweber et al. 2014). The illumination source was a tunable femtosecond laser (Insight, Spectra Physics or Chameleon, ...