This powerful approach to cutting and pasting DNA molecules is known as DNA cloning or recombinant DNA technology. When they act on a DNA molecule, restriction enzymes produce "blunt" ends when ...

Cloning DNA fragments using restriction enzymes is like flying from Seattle to New York via Phoenix. You arrive at your destination, but it takes longer than necessary to get there. Restriction sites, ...

Traditionally, cloning has been done using restriction enzyme sites on the multiple cloning site of a plasmid. And more recently, PCR cloning vectors have been popular because of their ease of use ...

We start with numerous cells from one person to obtain many sets of the chromosomes. Restriction enzymes will cut up, or cleave, DNA molecules wherever they encounter a specific sequence of bases.

The cutting and joining manipulations that underlies gene cloning are carried out by enzymes called restriction endonucleases (for cutting) and ligases (for joining). Nucleases degrade DNA molecules ...

Molecular biology has transitioned to a new era where precise genetic manipulation plays a critical role in research, medicine, and biotechnology. Among the vital tools in this field are cloning ...