The quality of your sequencing template greatly affects the success of the sequencing reaction. Sequencing by capillary electrophoresis is highly sensitive to sample contamination. Potential ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

The sequencing templates shown here have been produced by using the POLONY method. Right: an example of raw data from Pyrosequencing, a cyclic-array method. The identity of nucleotides used at ...

To enable parallel sequencing, the authors used a chip with thousands of nanoscale wells containing an immobilized DNA polymerase bound to a primed DNA template to be sequenced (Fig. 1).

However, minipreps are a necessary evil in research, and the number of sequencing templates to prepare never seems to dwindle. For example, a small lab running four sequencing gels a day with a ...

Customers can request to use these primers by ticking the appropriate box or boxes on the online sequencing request form in the section called MGS primers and by indicating in the column called Primer ...