The quality of your sequencing template greatly affects the success of the sequencing reaction. Sequencing by capillary electrophoresis is highly sensitive to sample contamination. Potential ...

The sequencing templates shown here have been produced by using the POLONY method. Right: an example of raw data from Pyrosequencing, a cyclic-array method. The identity of nucleotides used at ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

In Sanger sequencing, the DNA to be sequenced serves as a template for DNA synthesis. A DNA primer is designed to be a starting point for DNA synthesis on the strand of DNA to be sequenced.

Customers can request to use these primers by ticking the appropriate box or boxes on the online sequencing request form in the section called MGS primers and by indicating in the column called Primer ...