Sanger sequencing data must undergo a multi-step process to convert raw data into a usable state. “It’s a headache,” Dutken says. Exactly how sequencing data is processed will vary from lab ...

The Sanger sequencing method is based on the ... Automated sequencers have taken many of the steps of sequencing out of scientists' hands. But researchers still need to design sequencing primers ...

“There is significant debate within the diagnostics community regarding the necessity of confirming NGS variant calls by Sanger sequencing, considering that numerous laboratories report having 100% ...

After sequencing fragments of DNA to obtain reads, most genomic pipelines follow one of two steps. The reads can be de novo assembled ... but major advances include Sanger sequencing, sequencing by ...