A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

Primers We have several standard primers for the most common vectors and for bacterial 16S rDNA sequencing. These primers are available at no extra cost. If you want to use your own primers, take good ...

In Sanger sequencing, the DNA to be sequenced serves as a template for DNA synthesis. A DNA primer is designed to be a starting point for DNA synthesis on the strand of DNA to be sequenced.

Sanger sequencing is modeled after the natural process ... it is heated so that the two strands separate, and a synthetic primer is added to the mixture. The primer's sequence is complementary ...

All samples and primers must be dissolved in distilled water and must be provided at the concentrations below. For each sequencing reaction submit 10 μL of template and 10 μL of primer (templates and ...

You can find the sequencing primers suitable for your clone in the info sheet we send with your order. We can also verify the clones by Sanger sequencing with forward and reverse primers. For longer ...

Care should be taken quantifying your plasmid. The effects of template concentration on sequence quality can be seen in our technote: Sanger Sequencing sample primer concentration technote [PDF 524KB] ...