In Sanger sequencing, the DNA to be sequenced serves ... and T reactions are individually loaded into four lanes of a single gel and separated using gel electrophoresis, a method that separates ...

Sanger Sequencing, the workhorse of the human genome project ... It is strongly recommended that you run your products on a gel prior to sample submission. Plasmids - Cleaned plasmid DNA at 100ng/µl ...

Sanger sequencing relies on tagging the ends of various sizes of DNA fragments with complementary fluorescent nucleotides. Fragments are then separated by size using gel electrophoresis and the final ...

In addition, whereas traditional, gel-based Sanger sequencing and early machines could only generate 250 to 500 base pairs of DNA sequence per reaction, 750 to 1,000 base pairs of sequence can now ...