This powerful approach to cutting and pasting DNA molecules is known as DNA cloning or recombinant DNA technology. When they act on a DNA molecule, restriction enzymes produce "blunt" ends when ...

Cloning DNA fragments using restriction enzymes is like flying from Seattle to New York via Phoenix. You arrive at your destination, but it takes longer than necessary to get there. Restriction sites, ...

Traditionally, cloning has been done using restriction enzyme sites on the multiple cloning site of a plasmid. And more recently, PCR cloning vectors have been popular because of their ease of use ...

The cutting and joining manipulations that underlies gene cloning are carried out by enzymes called restriction endonucleases (for cutting) and ligases (for joining). Nucleases degrade DNA molecules ...