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Several well-established lipid-based in vitro DNA transfection reagents, such as InvivoGen's LipoGen and Invitrogen's Lipofectamine 2000 and Optifect—as well as their first-generation Lipofectin ...
Also, for ES cell assays it is important to include a 'mock' transfection control containing Lipofectamine 2000 alone, as this reagent may not be biologically inert. For example, we consistently ...